Biohydrogen production from cassava starch processing wastewater by thermophilic mixed cultures

نویسندگان

  • Sompong O-Thong
  • Adilan Hniman
  • Poonsuk Prasertsan
  • Tsuyoshi Imai
چکیده

Natural microbial consortia from hot spring samples were used to developed thermophilic mixed cultures for biohydrogen production from cassava starch processing wastewater (CSPW). Significant hydrogen production potentials were obtained from three thermophilic mixedculturesnamelyPK,SWandPRwithmaximumhydrogenproductionyieldsof249.3, 180 and 124.9 mL H2/g starch, respectively from raw cassava starch and 252.4, 224.4 and 165.4 mL H2/g starch, respectively fromgelatinized cassava starch. Acetic acid-ethanol and acetic-lactic acid type fermentation were observed in cassava starch fermentation, based on three thermophilic mixed cultures performance. The thermophilic mixed cultures PK, SW and PR exhibited the maximum hydrogen yield of 287, 264 and 232 mL H2/g starch in CSPW, respectively corresponding to53%, 48.7%and42.8%of the theoretical values. Phylogeneticanalysis of thermophilic mixed cultures revealed that members involved cassava starch degrading bacteria and hydrogen producers in both raw cassava starch andCSPWwere phylogenetically related to the Thermoanaerobacterium saccharolyticum, Thermoanaerobacterium thermosaccharolyticum, Anoxybacillus sp., Geobacillus sp. and Clostridium sp. Copyright a 2010, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights

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تاریخ انتشار 2011